Reporter

Part:BBa_K4799601:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtSRO1_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 309
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 309
    Illegal NheI site found at 3993
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 309
    Illegal BglII site found at 1975
    Illegal BamHI site found at 1958
    Illegal XhoI site found at 1100
    Illegal XhoI site found at 1132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 309
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 309
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
    Illegal NgoMIV site found at 2408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2208
    Illegal BsaI.rc site found at 2823
    Illegal SapI.rc site found at 2994


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References